Abstract
Background: Building a standard animal model of gastric and duodenal ulcers with specific pathological levels as in humans for the application of drug treatment trials is very necessary. So this study was aimed to build a Swiss mice model of gastric and duodenal ulcers using the stress method and evaluate the suitability of the built model.
Materials and method: Gastric ulcer was induced in mice for 3, 5, 7, 9 consecutive days by WIRS (Water Immersion Restraint Stress) method. The most optimal ulcer induction time was chosen based on macroscopic, microscopic assessment on mice stomachs and concentrations of biological biomakers related to inflammation including Glutathione and Malondialdehyde. The built model was evaluated for suitability by inducing ulcers under selected conditions to determine repeatability, then observing the lesions after 7, 14, 21 and 28 days to determine the self-healing status over time.
Results: After 9 days, the ulcerative status of the mouse gastroduodenal mucosa met all the evaluation criteria. Without treatment, it took 28 days for the gastroduodenal mucosa to return to normal physiological state.
Conclusion: The model has potential for application for the gastric ulcer treatment studies of research medicines in experimental animals.
| Published | 2025-06-25 | |
| Fulltext |
|
|
| Language |
|
|
| Issue | Vol. 15 No. 3 (2025) | |
| Section | Original Articles | |
| DOI | 10.34071/jmp.2025.3.15 | |
| Keywords | loét dạ dày tá tràng, WIRS, chuột nhắt chủng Swiss gastroduodenal ulcer, WIRS, Swiss mice |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2025 Hue Journal of Medicine and Pharmacy
Salari N, Darvishi N, Shohaimi S, Bartina Y, Ahmadipanah M, Salari HR, et al. The global prevalence of peptic ulcer in the world: A systematic review and meta-analysis. Indian J Surg 2022 Oct; 84(5):913-21.
Trần Văn Huy. Giáo trình Đại học Bệnh học nội khoa 1. Huế: Nhà xuất bản Đại học Huế; 2020.
Phạm Thị Vân Anh. Tác dụng điều trị loét dạ dày tá tràng của Dạ dày Hoàn Bà Giằng trên thực nghiệm. Bộ môn Dược lý - Đại học Y Hà Nội, Hà Nội 2018.
Almasaudi SB, El-Shitany NA, Abbas AT, Abdel-dayem UA, Ali SS, Al Jaouni SK, et al. Antioxidant, anti‐inflammatory, and antiulcer potential of manuka honey against gastric ulcer in rats. Oxid Med Cell Longev 2016; 2016(1):3643824.
Bi WP, Man HB, Man MQ. Efficacy and safety of herbal medicines in treating gastric ulcer: a review. World Journal of Gastroenterol 2014 Dec 7; 20(45):17020.
Yao K, Cao L, Ding H, Gao Y, Li T, Wang G, et al. Increasing Aspartoacylase in the Central Amygdala: The Common Mechanism of Gastroprotective Effects of Monoamine-Based Antidepressants Against Stress. Frontiers Pharmacol 2022 Feb 25; 13:823291.
Xu Y, Jia J, Xie C, Wu Y, Tu W. Transient receptor potential ankyrin 1 and substance P mediate the development of gastric mucosal lesions in a water immersion restraint stress rat model. Digestion 2018 Mar 20; 97(3):228-39.
Elgohary R, Abdelsalam RM, Abdel-Salam OM, Khattab MM, Salem NA, El-Khyat ZA, et al. Protective effect of cannabinoids on gastric mucosal lesions induced by water immersion restrain stress in rats. Iran J Basic Med Sci 2021 Sep; 24(9):1182.
Adinortey MB, Ansah C, Galyuon I, Nyarko A. In vivo models used for evaluation of potential antigastroduodenal ulcer agents. Ulcers 2013; 2013(1):796405.
Ren S, Wei Y, Wang R, Wei S, Wen J, Yang T, Chen X, Wu S, Jing M, Li H, Wang M. Rutaecarpine ameliorates ethanol-induced gastric mucosal injury in mice by modulating genes related to inflammation, oxidative stress and apoptosis. Front Pharmacol. 2020 Nov 26; 11:600295.
Bộ Y tế. Hướng dẫn quy trình kỹ thuật chuyên ngành Giải phẫu bệnh, tế bào học. Hà Nội: Nhà xuất bản Y học; 2016.
Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959 May 1; 82(1):70-7.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979 Jun 1; 95(2):351-8.
Kuroiwa M, Sugiyama S, Goto H, Tsukamoto Y, Nakazawa S, Ozawa T. The role of mucosal prostaglandin levels in healing of water immersion-induced gastric ulcers in rats. Scand J Gastroenterol. 1990 Jan 1; 25(1):59-65.
Adinortey MB, Ansah C, Galyuon I, Nyarko A. In vivo models used for evaluation of potential antigastroduodenal ulcer agents. Ulcers 2013; 2013(1):796405.
Choudhary P, Roy T, Chatterjee A, Mishra VK, Pant S, Swarnakar S. Melatonin rescues swim stress induced gastric ulceration by inhibiting matrix metalloproteinase-3 via down-regulation of inflammatory signaling cascade. Life Sci 2022 May 15; 297:120426.
Kim YJ, Kim EH, Hahm KB. Oxidative stress in inflammation‐based gastrointestinal tract diseases: Challenges and opportunities. J Gastroenterol Hepatol 2012 Jun; 27(6):1004-10.
Woźniak B, Woźniak A, Kasprzak HA, Drewa G, Mila-Kierzenkowska C, Drewa T, Planutis G. Lipid peroxidation and activity of some antioxidant enzymes in patients with glioblastoma and astrocytoma. J Neurooncol 2007 Jan; 81:21-6.
Weydert CJ, Cullen JJ. Measurement of superoxide dismutase, catalase and glutathione peroxidase in cultured cells and tissue. Nat Protoc 2010 Jan; 5(1):51-66.
Elsaed WM, Alahmadi AM, Al-Ahmadi BT, Taha JA, Tarabishi RM. Gastroprotective and antioxidant effects of fluvoxamine on stress-induced peptic ulcer in rats. J Taibah Univ Med Sci 2018 Oct 1; 13(5):422-31.