Indexing
CÁC SỐ TỪ 2011-2023
Tạp chí Y Dược Học
Abstract
Background: Gut microbiota dysbiosis contributes to chronic inflammation, promoting the progression of adenomatous polyps and colorectal cancer (CRC). This study aimed to: (1) Evaluate the efficiency of gut microbiota DNA extraction from fecal samples using the QIAamp PowerFecal Pro DNA Kit; (2) Investigate the gut microbiota in three representative fecal samples (derived from patients with adenomatous polyps, CRC, and healthy controls) by sequencing the V3 - V4 region of the 16S rRNA gene. Methods: Thirty fecal samples (10 controls, 10 adenomatous polyps, 10 CRC) were subjected to DNA extraction using the QIAamp PowerFecal Pro DNA Kit. To characterise the gut microbiota, one sample was randomly selected from each group and sequenced on the Illumina MiSeq platform. Results: High-yield DNA extraction was achieved in all 30 samples, median DNA concentration was 252.1 (IQR: 162.0 - 311.9) ng/μL, with good purity and specific PCR amplification (550 bp). Analysis of gut microbiota in three representative samples revealed an increased Firmicutes/Bacteroidota ratio in the CRC sample compared with the adenomatous polyp and control sample; Prevotella predominated in the adenomatous polyp sample; Fusobacterium nucleatum, Enterococcus faecalis, and Streptococcus gallolyticus were detected in the CRC sample but were absent in the control sample. Conclusion: DNA extraction from fecal samples was successful, providing sufficient quality for 16S rRNA sequencing to analyze the gut microbiota in patients with adenomatous polyps and colorectal cancer.
| Published | 2026-03-30 | |
| Fulltext |
|
|
| Language |
|
|
| Issue | Vol. 16 No. 1 (2026) | |
| Section | Original Articles | |
| DOI | 10.34071/jmp.2025.6.737 | |
| Keywords | Fecal DNA extraction, gut microbiota sequencing, adenomatous polyps, colorectal cancer Tách chiết DNA từ mẫu phân, giải trình tự hệ vi sinh đường ruột, polyp tuyến, ung thư đại trực tràng |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2026 Hue Journal of Medicine and Pharmacy
Kim J, Gunathilake M, Yeo HY, Oh JH, Kim BC, Han N, et al. Fecal Microbial Dysbiosis is Associated with Colorectal Cancer Risk in a Korean Population. Cancer Res Treat. 2024 Jul 26;57(1):198-211.
Deng D, Zhao L, Song H, Wang H, Cao H, Cui H, et al. Microbiome analysis of gut microbiota in patients with colorectal polyps and healthy individuals. Sci Rep. 2025 Dec 1;15(1):7126.
Périchon B, Lichtl-Häfele J, Bergsten E, Delage V, Trieu-Cuot P, Sansonetti P, et al. Detection of Streptococcus gallolyticus and Four Other CRC-Associated Bacteria in Patient Stools Reveals a Potential “Driver” Role for Enterotoxigenic Bacteroides fragilis. Front Cell Infect Microbiol. 2022 Mar 11;12:794391.
Rebersek M. Gut microbiome and its role in colorectal cancer. BMC Cancer. BioMed Central Ltd; 2021 Dec;21:1325.
Fernández-Pato A, Sinha T, Gacesa R, Andreu-Sánchez S, Gois MFB, Gelderloos-Arends J, et al. Choice of DNA extraction method affects stool microbiome recovery and subsequent phenotypic association analyses. Sci Rep. 2024 Dec 1;14(1):3911.
Qiagen. QIAamp ® PowerFecal ® Pro DNA Kit Handbook. 2020.
Rezasoltani S, Aghdaei HA, Jasemi S, Gazouli M, Dovrolis N, Sadeghi A, et al. Oral Microbiota as Novel Biomarkers for Colorectal Cancer Screening. Cancers (Basel). 2023 Jan 1;15(1):192.
Illumina. 16S Metagenomic Sequencing Library Preparation. Preparing 16S Ribosomal RNA Gene Amplicons for the Illumina MiSeq System.
Lim MY, Park YS, Kim JH, Nam Y Do. Evaluation of fecal DNA extraction protocols for human gut microbiome studies. BMC Microbiol. 2020 Jul 17;20(1):212.
Kadhim FJ, Aziz ZS, Ibrahim KS. Gut Microbiome Profiles in Colorectal Cancer Patients in Iraq. Microbiol Res (Pavia). 2025 Jan 1;16(1):22.
Dong Y, Zhang K, Wei J, Ding Y, Wang X, Hou H, et al. Gut microbiota-derived short-chain fatty acids regulate gastrointestinal tumor immunity: a novel therapeutic strategy? Frontiers in Immunology. Frontiers Media S.A. 2023 Apr;14:1158200.
El Leithy AA, Youssef ASED, Nassar A, Aziz RK, Khaled NM, Mahrous MT, et al. Long-read 16S rRNA amplicon sequencing reveals microbial characteristics in patients with colorectal adenomas and carcinoma lesions in Egypt. Gut Pathog. 2025 Dec 1;17(1):8.
Shah AB, Baiseitova A, Zahoor M, Ahmad I, Ikram M, Bakhsh A, et al. Probiotic significance of Lactobacillus strains: a comprehensive review on health impacts, research gaps, and future prospects. Gut Microbes. Taylor and Francis Ltd. 2024 Jan-Dec;16(1):2431643.
Lo CH, Wu DC, Jao SW, Wu CC, Lin CY, Chuang CH, et al. Enrichment of Prevotella intermedia in human colorectal cancer and its additive effects with Fusobacterium nucleatum on the malignant transformation of colorectal adenomas. J Biomed Sci. 2022 Dec 1;29(1):88.
Ou S, Wang H, Tao Y, Luo K, Ye J, Ran S, et al. Fusobacterium nucleatum and colorectal cancer: From phenomenon to mechanism. Frontiers in Cellular and Infection Microbiology. Frontiers Media S.A. 2022 Nov 29;12:1020583.
Zhang L, Liu J, Deng M, Chen X, Jiang L, Zhang J, et al. Enterococcus faecalis promotes the progression of colorectal cancer via its metabolite: biliverdin. J Transl Med. 2023 Feb 2;21(1):72.
Eldegla HE, Abdel-Wahhab M, Moemen D. Association between Streptococcus gallolyticus and colorectal cancer in Mansoura University hospitals. Egyptian Journal of Basic and Applied Sciences. 2021;8(1):397-406.
Dadgar-Zankbar L, Shariati A, Bostanghadiri N, Elahi Z, Mirkalantari S, Razavi S, et al. Evaluation of enterotoxigenic Bacteroides fragilis correlation with the expression of cellular signaling pathway genes in Iranian patients with colorectal cancer. Infect Agent Cancer. 2023 Dec 1;18(1):48.