Abstract
Introduction: A common cause of failure in endodontic treatment is bacterial penetration into the root canal system through microleakage after obturation, particularly when post-space preparation is required.
Objective: To evaluate bacterial leakage in post-space prepared to half the root canal length using different methods following root canal obturation.
Subjects and Methods: An in vitro study, without a control group, was conducted on 40 mandibular premolars, which were divided into 4 groups according to the obturation method and the timing of post-space preparation. Bacterial leakage was assessed by the turbidity of the BHI solution in each group, and Gram staining was performed if turbidity appeared.
Results: Group 1 exhibited a delayed bacterial leakage time compared to Group 2. Additionally, Group 3 showed a later leakage time than Group 4. However, Group 1 had an earlier bacterial leakage time compared to Group 3, and Group 2 had an earlier leakage time than Group 4. The differences were statistically significant (p < 0.05). Gram-positive bacteria (90%) and Gram-negative bacteria (10%) were identified in the group with lateral compaction obturation. In the group with thermoplasticized Gutta Percha obturation, only Gram-positive bacteria (100%) were found.
Conclusion: Immediate post-space preparation resulted in a significantly delayed bacterial leakage compared to delayed post-space preparation. Post-space preparation in teeth obturated with thermoplasticized Gutta Percha resulted in a significantly delayed bacterial leakage compared to those obturated using the lateral compaction technique.
| Published | 2025-08-30 | |
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| Issue | Vol. 15 No. 4 (2025) | |
| Section | Original Articles | |
| DOI | 10.34071/jmp.2025.4.19 | |
| Keywords | Bacterial leakage, post-space preparation, obturation |
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright (c) 2025 Hue Journal of Medicine and Pharmacy
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